桂耀庭,医学博士,研究员

 

一、教育与工作经历

1990.09 - 1993.07  同济医科大学生殖医学专业博士研究生
1993.07 - 1996.12  深圳市第二人民医院助理研究员
1996.12 - 1999.10  美国北卡罗来纳大学 (University of North Carolina at Chapel Hill) 医学院博士后研究人员
1999.10 - 2003.07  加拿大马尼托巴大学 (University of Manitoba) 医学院高级博士后研究人员 (Research Associate)
2003.07 ----     北京大学深圳医院男性生殖实验室主任、副研究员、研究员
2006.12 ----     深圳市男性生殖与遗传重点实验室副主任、主任
2008.08 ----     广东省男性生殖与遗传重点实验室副主任、主任
2008.12 ----     深圳北京大学香港科技大学医学中心泌尿外科研究所副所长、所长















 


二、 主持的主要科研项目

1.  2007年国家自然科学基金项目:新基因TSC21在小鼠和人体睾丸组织的表达特征与功能研究(编号:30770810)2008201029万元;

2.  2009年国家自然科学基金项目:附睾蛋白酶抑制因子(EPPIN) 基因转录调控的分子机理(编号:30971636)2010201233万元;

3.  2011年国家重大科学研究计划项目:精子遗传信息稳定传递的分子机理 (编号:2011CB944303)子课题项目负责人20112015210万元;

4.  2012国家自然科学基金项目:雄激素调控泛素结合酶UBE2B表达的分子机理及其生物学意义(编号:31271244)2013201672万元;

5.  2014国家自然科学基金项目:Repro29雄性不育小鼠致病基因的鉴定和功能研究 (编号:31471344)2015201882万元;

6.  2010年深圳市重点实验室提升计划项目:采用大规模测序方法探讨男性不育症的分子机理(编号:CXB201005250017A)20112013100万元;

7.  2011深圳市重点实验室提升计划项目四种雄性不育小鼠致病基因的定位克隆及功能研究(编号:CXB201104220045A)20122014160万元;

8.  2011年深圳市卫生与人口计划生育委员会重点学科建设项目:泌尿外科重点学科群(生殖与遗传医学重点实验室),20122016300万元。

9.  2012深圳市重点实验室提升计划项目:三个染色质重塑相关基因在膀胱癌发生发展过程中的作用及其分子机制(编号:ZDSY20120615 154448514)2013201580万元;

10.  2013年深圳市基础研究计划项目:雄激素及其受体调控精子发生的分子机制(JCYJ201304021131312022014201650万元;

11.  2014年广东省重点实验室提升计划项目:生精障碍发生的分子遗传学机制(编号:2014B030301001)2015-2017300 万元;

12.  2017年广东省重点实验室提升计划项目:原发性无精子症相关基因的筛选与功能研究(编号:2017B030314074)2018-202080 万元;

13.  2017年深圳市基础研究(学科布局)项目:原发性无精子症相关基因的筛选与功能研究(编号:JCYJ20170413100245260)2018-2020200万元。



三、主要论文(*通讯作者)

1.     Gui Y, Zhang JL, Yuan LW, Lessey BA. Regulation of Hoxa-10 and its expression in normal and abnormal endometrium. Molecular Human Reproduction. 1999; 5(9): 866-873.

2.     Gui Y, Murphy LJ.  Insulin-like growth factor (IGF) binding protein-3 (IGFBP-3) binds to fibronectin: Demonstration of IGF-I/IGFBP-3/FN ternary complexes in human plasma.  Journal of Clinical Endocrinology and Metabolism. 2001; 86(5): 2104-10

3.     Gui Y and Murphy LJ. Interaction of IGFBP-3 with latent transforming growth factor-beta binding protein-1. Molecular and Cellular Biochemistry. 2003; 250:189-195

4.     Gui Y, Silha JV and Murphy LJ. Changes in adipokine expression during food-deprivation in the mouse and the relationship to fasting- induced insulin resistance Canadian Journal of Physiology and Pharmacology. 2003; 81:979-985

5.     Gui Y, Silha JV and Murphy LJ. Sexual dimorphism and hormonal regulation of resistin, adiponectin and leptin expression in the mouse. Obesity Research. 2004; 12: 1481-1491

6.     Gui Y, Cai ZM, Silha JV, and Murphy LJIncreased white adipose tissue in mouse diestrous stage: Relationship with the expression of PPAR g and RXRa. Canadian Journal of Physiology and Pharmacology. 2006; 84:887-892.

7.     Zhou YC, Qin DN, Tang AF, Zhou DR, Qin J, Yan B, Diao RY, Jiang ZM, Cai ZM and Gui Y*. Developmental Expression Pattern of a Novel Gene TSG23/Tsg23 Suggests Its Role in Spermatogenesis. Molecular Human Reproduction. 2009; 15(4): 223-230.

8.     Chen J, Wang Y, Wei B, Lai Y, Yan Q, Gui Y*, Cai Z. Functional expression of ropporin in human testis and ejaculated spermatozoa. Journal of Andrology. 2011; 32(1):26-32.

9.     Xu X, Nagarajan H, Lewis NE, Pan S, Cai Z, Liu X, Chen W, Xie M, Wang W, Hammond S, Andersen MR, Neff N, Passarelli B, Koh W, Fan HC, Wang J, Gui Y, Lee KH, Betenbaugh MJ, Quake SR, Famili I, Palsson BO, Wang J. The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line. Nature Biotechnology. 2011; 29(8):735-41.

10.     Gui Y, Guo G, Huang Y, Hu X, Tang A, Gao S, Wu R, Chen C, Li X, Zhou L, He M, Li Z, Sun X, Jia W, Chen J, Yang S, Zhou F, Zhao X, Wan S, Ye R, Liang C, Liu Z, Huang P, Liu C, Jiang H, Wang Y, Zheng H, Sun L, Liu X, Jiang Z, Feng D, Chen J, Wu S, Zou J, Zhang Z, Yang R, Zhao J, Xu C, Yin W, Guan Z, Ye J, Zhang H, Li J,Kristiansen K, Nickerson ML, Theodorescu D, Li Y, Zhang X, Li S, Wang J, Yang H, Wang J, Cai ZM. Frequent mutations of chromatin remodeling genes in transitional cell carcinoma of the bladder. Nature Genetics. 2011; 43(9):875-8.

11.     Liu XY, Fu J, Cai ZM, Gui Y*. A novel nonsense mutation in the androgen receptor gene causes the complete androgen insensitivity syndrome. Journal of Andrology. 2012; 33(3):357-60

12.     Guo G, Gui Y (Co-first author),  Gao S, Tang A, Hu X, Huang Y, Jia W, Li Z, He M, Sun L, Song P, Sun X, Zhao X, Yang S, Liang C, Wan S, Zhou F, Chen C, Zhu J, Li X, Jian M, Zhou L, Ye R, Huang P, Chen J, Jiang T, Liu X, Wang Y, Zou J, Jiang Z, Wu R, Wu S, Fan F, Zhang Z, Liu L, Yang R, Liu X, Wu H, Yin W, Zhao X, Liu Y, Peng H, Jiang B, Feng Q, Li C, Xie J, Lu J, Kristiansen K, Li Y, Zhang X, Li S, Wang J, Yang H, Cai ZM, Wang J. Frequent mutations of genes encoding ubiquitin-mediated proteolysis pathway components in clear cell renal cell carcinoma. Nature Genetics. 2012; 44(1):17-9.

13.     Hu Z, Xia Y, Guo X, Dai J, Li H, Hu H, Jiang Y, Lu F, Wu Y, Yang X, Li H, Yao B, Lu C, Xiong C, Li Z, Gui Y, Liu J, Zhou Z, Shen H, Wang X, Sha J. A genome-wide association study in Chinese men identifies three risk loci for non-obstructive azoospermia. Nature Genetics. 2012; 44(2):183-6.

14.     Xu X, Hou Y, Yin X, Bao L, Tang A, Song L, Li F, Tsang S, Wu K, Wu H, He W, Zeng L, Xing M, Wu R, Jiang H, Liu X, Cao D, Guo G, Hu X, Gui Y, Li Z, Xie W, Sun X, Shi M, Cai Z, Wang B, Zhong M, Li J, Lu Z, Gu N, Zhang X, Goodman L, Bolund L,Wang J, Yang H, Kristiansen K, Dean M, Li Y, Wang J. Single-cell exome sequencing reveals single-nucleotide mutation characteristics of a kidney tumor. Cell. 2012; 148(5): 886-95.

15.     Mou L, Wang Y, Li H, Huang Y, Jiang T, Huang W, Li Z, Chen J, Xie J, Liu Y, Jiang Z, Li X, Ye J, Cai Z, Gui Y*. A dominant-negative mutation of HSF2 associated with idiopathic azoospermia.  Human Genetics. 2013; 132(2):159-65.

16.     Wang XN, Li ZS, Ren Y, Jiang T, Wang YQ, Chen M, Zhang J, Hao JX, Wang YB, Sha RN, Huang Y, Liu X, Hu JC, Sun GQ, Li HG, Xiong CL, Xie J, Jiang ZM, Cai ZM, Wang J, Wang J, Huff V, Gui Y*, Gao F. The Wilms tumor gene, Wt1, is critical for mouse spermatogenesis via regulation of Sertoli cell polarity and is associated with non-obstructive azoospermia in humans. PLoS Genetics. 2013; 9(8): e1003645.

17.     Mou L, Zhang Q, Wang Y, Zhang Q, Sun L, Li C, Huang W, Yuan Y, Duan Y, Diao R, Jiang Z, Ye J, Cai Z, Gui Y*. Identification of Ube2b as a novel target of androgen receptor in mouse Sertoli cells. Biology of Reproduction. 2013; 89(2):32: 1-11.

18.     Guo G, Sun X, Chen C, Wu S, Huang P, Li Z, Dean M, Huang Y, Jia W, Zhou Q, Tang A, Yang Z, Li X, Song P, Zhao X, Ye R, Zhang S, Lin Z, Qi M, Wan S, Xie L, Fan F, Nickerson ML, Zou X, Hu X, Xing L, Lv Z, Mei H, Gao S, Liang C, Gao Z, Lu J, Yu Y, Liu C, Li L, Fang X, Jiang Z, Yang J, Li C, Zhao X, Chen J, Zhang F, Lai Y, Lin Z, Zhou F, Chen H, Chan HC, Tsang S, Theodorescu D, Li Y, Zhang X, Wang J, Yang H, Gui Y*, Wang J, Cai Z. Whole-genome and whole-exome sequencing of bladder cancer identifies frequent alterations in genes involved in sister chromatid cohesion and segregation. Nature Genetics. 2013; 45(12): 1459-63.

19.     Han Y, Liu Y, Zhang H, Wang T, Diao R, Jiang Z, Gui Y* and Cai Z. Hsa-miR-125b suppresses bladder cancer development by down-regulating oncogene SIRT7 and oncogenic long non-coding RNA MALAT1. FEBS Letters. 2013; 587(12): 3875–3882.

20.     Wu S, Huang P, Li C, Huang Y, Li X, Wang Y, Chen C, Lv Z, Tang A, Sun X, Lu J,Li W, Zhou J, Gui Y, Zhou F, Wang D, Cai Z. Telomerase reverse transcriptase gene promoter mutations help discern the origin of urogenital tumors: a genomic and molecular study. European Urology. 2014; 65(2):274-7.

21.     Yang L, Wang Y, Zhang Q, Lai Y, Li C, Zhang Q, Huang W, Duan Y, Jiang Z, Li X, Cai Z, Mou L, Gui Y*. Identification of Hsf1 as a novel androgen receptor-regulated gene in mouse Sertoli cells. Molecular Reproduction and Development. 2014; 81(6): 514-23.

22.     Hu Z, Li Z, Yu J, Tong C, Lin Y, Guo X, Lu F, Dong J, Xia Y, Wen Y, Wu H, Li H, Zhu Y, Ping P, Chen X, Dai J, Jiang Y, Pan S, Xu P, Luo K, Du Q, Yao B, Liang M, Gui Y, Weng N, Lu H, Wang Z, Zhang F, Zhu X, Yang X, Zhang Z, Zhao H, Xiong C, Ma H, Jin G, Chen F, Xu J, Wang X, Zhou Z, Chen ZJ, Liu J, Shen H, Sha J. Association analysis identifies new risk loci for non-obstructive azoospermia in Chinese men. Nature Communications. 2014; 5:3857.

23.     Diao R, Fok KL, Chen H, Yu MK, Duan Y, Chung CM, Li Z, Wu H, Li Z, Zhang H, JiZ, Zhen W, Ng CF, Gui Y, Cai Z, Chan HC. Deficient human β-defensin 1 underlies male infertility associated with poor sperm motility and genital tract infection. Science Translational Medicine. 2014; 6(249):249ra108.

24.     Li Z, Huang Y, Li H, Hu J, Liu X, Jiang T, Sun G, Tang A, Sun X, Qian W, Zeng Y, Xie J, Zhao W, Xu Y, He T, Dong C, Liu Q, Mou L, Lu X, Lin Z, Wu S, Gao S, Guo G, Feng Q, Li Y, Zhang X, Wang J, Yang H, Wang J, Xiong C, Cai Z, Gui Y*. Excess of rare variants in genes that are key epigenetic regulators of spermatogenesis in the patients with non-obstructive azoospermia. Scientific Reports, 2015; 5: 8785.

25.     Mou L, Zhang Q, Diao R, Cai Z, Gui Y*. A functional variant in the UBE2B gene promoter is associated with idiopathic azoospermia. Reproductive Biology and Endocrinology. 2015; 13:79.  

26.     Mou L, Xie N, Yang L, Liu Y, Diao R, Cai Z, Li H, Gui Y*. A Novel Mutation of DAX-1 Associated with Secretory Azoospermia. PLoS One. 2015; 10(7): e0133997.  

27.     Li Y, Lin S, Luo M, Guo H, Chen J, Ma Q, Gu Y, Jiang Z, Gui Y*. FAM170B, a novel acrosomal protein involved in fertilization in mice. Molecular Reproduction and Development. 2015; 82(10):787-96.

28.     Guo H, Li Y, Luo M, Lin S, Chen J, Ma Q, Gu Y, Jiang Z, Gui Y*. Androgen receptor binding to an androgen-responsive element in the promoter of the Srsf4 gene inhibits its expression in mouse Sertoli cells. Molecular Reproduction and Development. 2015; 82(12): 976-85.

29.     Luo M, Li Y, Guo H, Lin S, Chen J, Ma Q, Gu Y, Jiang Z, Gui Y*. Protein Arginine Methyltransferase 6 Involved in Germ Cell Viability during Spermatogenesis and Down-Regulated by the Androgen Receptor. International Journal of Molecular Sciences. 2015; 16(12):29467-81.  

30.     Du Y, Li M, Chen J, Duan Y, Wang X, Qiu Y, Cai Z, Gui Y*, Jiang H. Promoter targeted bisulfite sequencing reveals DNA methylation profiles associated with low sperm motility in asthenozoospermia. Human Reproduction. 2016; 31(1):24-33.

31.     Tang A, Huang Y, Li Z, Wan S, Mou L, Yin G, Li N, Xie J, Xia Y, Li X, Luo L, hang J, Chen S, Wu S, Sun J, Sun X, Jiang Z, Chen J, Li Y, Wang J, Wang J, Cai , Gui Y*. Analysis of a four generation family reveals the widespread equence-dependent maintenance of allelic DNA methylation in somatic and germ cells. Scientific Reports. 2016; 6:19260.  

32.     Liu X, Li Z, Su Z, Zhang J, Li H, Xie J, Xu H, Jiang T, Luo L, Zhang R, Zeng X, Xu H, Huang Y, Mou L, Hu J, Qian W, Zeng Y, Zhang X, Xiong C, Yang H, Kristiansen K, Cai Z, Wang J, Gui Y*. Novel Y-chromosomal microdeletions associated with non-obstructive azoospermia uncovered by high throughput sequencing of sequence-tagged sites (STSs). Scientific Reports. 2016; 6:21831.

33.     Ma Q, Li Y, Guo H, Li C, Chen J, Luo M, Jiang Z, Li H, Gui Y*. A novel missense mutation in USP26 gene is associated with nonobstructive azoospermia. Reproductive Sciences. 2016; 23(10):1434-41.

34.     Chen J, Gu Y, Zhang Z, Zheng W, Yang L, Huang W, Lin S, Li Y, Guo H, Luo M, Ma Q, Jiang Z, Tang A, Gui Y*. Deficiency of SPATA46, a novel nuclear membrane protein, causes subfertility in male mice. Biology of Reproduction. 2016; 95(3):58, 1-10.

35.     Li Y, Li C, Lin S, Yang B, Huang W, Wu H, Chen Y, Yang L, Luo M, Guo H, Chen J, Wang T, Ma Q, Gu Y, Mou L, Jiang Z, Xia J, Gui Y*. A nonsense mutation in Ccdc62 gene is responsible for spermiogenesis defects and male infertility in repro29/repro29 mice. Biology of Reproduction. 2017; 96(3):587-597.  

36.     Deng Q, Wu Y, Zhang Z, Wang Y, Li M, Liang H, Gui Y*. Androgen Receptor Localizes to Plasma Membrane by Binding to Caveolin-1 in Mouse Sertoli Cells. International Journal of Endocrinology. 2017; 2017:3985916.

37.     Huang X, Zhuang C, Zhuang C, Xiong T, Li Y, Gui Y*. An enhanced hTERT promoter-driven CRISPR/Cas9 system selectively inhibits the progression of bladder cancer cells. Molecular Biosystems. 2017; 13(9):1713-1721

38.     Deng Q, Zhang Z, Wu Y, Yu WY, Zhang J, Jiang ZM, Zhang Y, Liang H, Gui Y*. Non-genomic action of androgens is mediated by rapid phosphorylation and regulation of androgen receptor trafficking. Cellular Physiology and Biochemistry. 2017; 43(1):223-236.

39.     Ma Q, Li Y, Luo M, Guo H, Lin S, Chen  J, Du Y, Jiang Z, Gui Y*. The expression characteristics of FAM71D and its association with sperm motility. Human Reproduction, 2017, 32(11):2178-2187.

40.     Zhuang C, Huang X, Zhuang C, Luo X, Zhang X, Cai Z, Gui Y*. Synthetic regulatory RNAs selectively suppress the progression of bladder cancer. Journal of Experimental & Clinical Cancer Research. 2017; 36(1):151.

41.     Li C, Yang B, Pan P, Ma Q, Wu Y, Zhang Z, Guo X, Ye J, Gui Y*. MicroRNA-130a inhibits spermatogenesis by directly targeting androgen receptor in mouse Sertoli cells. Molecular Reproduction & Development. 2018 Sep 6. doi: 10.1002/mrd.23058.

 

四、 科技成果奖

1. 桂耀庭、唐爱发、蔡志明、余振东、郭新、张立兵、张健荣、李贤新、石敏、李世勤、朱辉、于洁、房家智:精子发生相关基因的筛选和功能研究,2006年深圳市首届科技创新奖;

2.  蔡志明、桂耀庭、于洁、肖晓素、唐爱发、郭新、崔光辉、秦洁:生精障碍发生的分子机理及治疗途径探索,2009年中华医学科技三等奖;

3. 蔡志明、桂耀庭、黄毅、吴松、侯勇、李泽松、韩永华、孙小娟、黄卫人、李英睿:泌尿男生殖系肿瘤多组学研究及其转化应用,2014年中华医学科技二等奖;

4. 蔡志明、桂耀庭、徐讯、李泽松、李贤新、黄毅:泌尿生殖系肿瘤多组学研究及其转化应用,2014年深圳市自然科学一等奖;

5. 蔡志明、王俊、桂耀庭、徐讯、郭广武、韩永华、黄毅、张秀清、周芳坚、吴松、刘宇辰、李贤新、周亮、唐爱发、杨焕明:泌尿系统恶性肿瘤的基因组学研究,2015年广东省自然科学一等奖;

6. 蔡志明、王俊、桂耀庭、唐爱发、吴松、黄毅、徐讯、郭广武、刘宇辰、韩永华、李贤新、周亮、周芳坚、张秀清、杨焕明:泌尿系统恶性肿瘤的基因组学研究,2015年教育部高等学校自然科学二等奖。

 

五、研究方向

1.         精子发生与生精障碍的分子生物学机制;

2.         肾癌和膀胱癌发生的分子生物学机制;

 




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